Size  100ug
Cat code  200256
Species Cross-reactivity  H
Key Application  WB,IHC,FC
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG1
Molecular Weight  143kDa
Sensitivity  This antibody detects endogenous levels SMC1A and does not cross-react with related proteins.
Immunogen  Purified recombinant human SMC1A protein fragments expressed in E.coli.
UniProt Number  Q14683
GeneBank ID  NM_006306
Subcellular location  Nucleus. Chromosome
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername  SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; KIAA0178; MGC138332; SMC1alpha; DKFZp686L19178
Gene_symbol  SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; KIAA0178; MGC138332; SMC1alpha; DKFZp686L19178
Summary  Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1L2 or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation.
UniPort summary  Function  Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interac tion with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
Application Images :  
Western blot detection of SMC1A in HelaNE,Jurkat&A431 cell lysates using SMC1A antibody (1:1000 diluted).
Predicted band size: 143KDa.
Observed band size: 143KDa.
IHC of paraffin-embedded human colon using anti-SMC1A (N-terminus) diluted 1/500-1/1000.
Flow Cytometry analysis of HeLa cells stained with SMC1A (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG.